Examination of glucose
1) Glucose as a reducing agent
Required: 2% glucose-solution; Fehling-solution I + II; BB; test-tube; - holder
Procedure:
Fill 2 [ml] of glucose-solution into a small test-tube and add further 2 [ml] of each Fehling I and II. Warm carefully until colour change.
Attention: Fehling solution is strong basic. To avoid damages never hold the open end of the t.-t. in the direction of your neighbour! - Do not boil! (delay in boiling)
Observations? - Conclusions?
2) Chain and ring structure
Required: 2% glucose-solution; Schiff`s reagent; ethanal; t.-t.
Procedure:
Fill 2 [ml] of ethanal into a t.-t. and add dropwise Schiff`s reagent.
Give 2 [ml] of glucose-solution into another t.-t. and add again dropwise Schiff`s reagent.
Observations? - Conclusions?
3) Enzyme-Test (GOD-Test)
Required: 2% -solutions of each glucose, fructose and saccharose; glucotest-strips, 3 small beakers
Procedure:
Fill into each beaker a small amount of sugar solution (beaker 1 with sugar 1 etc.)
Hold for a short moment a Glucotest-strip into the solution.
Observations? - Result?