Saccharose

1) Fehling-Test

Try the Fehling-test with the given 2% saccharose-solution! – A disaccharide consists of two rings, one of them here is glucose.

What do you conclude from the observation above?


2) Hydrolysis by acid

Required: 2% saccharose-solution; cone, hydrochloric acid; dil. sodiumhydroxide-solution; GOD-Testpaper; Fehling I + II; resorcin-reagent; t.t.; BB

Fill ca. two ml of saccharose-solution into a test-tube and add carefully 2-3 drops of the acid. Boil for two minutes and allow to cool (water-cool from the tap).
Divide into two portions and neutralize by addition of a few ml of sodiumhydroxide-solution. Check with pH-paper! Test the acidic half with resorcin-reagent! Test the neutralized half with GOD-paper and with Fehling-solution!

Conclusions?


3) Enzymatic fission

Required: 2% saccharose-solution; yeast; GOD-paper; small beaker

Put together equal amounts of saccharose-solution and yeast-suspension. Check immediately with GOD-paper. Check again after 10 minutes.

Conclusions?


Maltose

4) Fehling-Test with maltose

Try the Fehling-test with the given 2% maltose-solution!

Observations? - Conclusions?

5) Hydrolysis

Analogue to 2)

Observations? - Conclusions?